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KMID : 0545120100200071107
Journal of Microbiology and Biotechnology
2010 Volume.20 No. 7 p.1107 ~ p.1113
Evaluation of Various PCR Assays for the Detection of Emetic Toxin-Producing Bacillus cereus
Kim Jung-Beom

Kim Jae-Myung
Park Yong-Bae
Han Jeong-A
Lee Soon-Ho
Kwak Hyo-Sun
Hwang In-Gyun
Yoon Mi-Hye
Lee Jong-Bok
Oh Deog-Hwan
Abstract
Because conventional methods for detecting emetic-toxinproducing B. cereus are laborious and costly, various PCR assays, which are easy and cheap, have recently been reported. Therefore, this study estimated and compared the ability of various PCR assays to detect emetic-toxinproducing B. cereus strains isolated in Korea. The PCR assays were performed on 160 B. cereus strains, including 40 emetic-toxin-producing strains. Although the speciesspecific PCR assays were all shown to be highly specific, the sensitivities varied greatly. The accuracies of the primers were 97.5% (CER), 95.6% (EM1), 96.3% (RE234), 89.4% (CES), and 83.1% (Ces3R/CESR2). Moreover, the CER primer had a higher sensitivity (100%) than all the other primers tested, and a specificity of 96.7%. Thus, the CER primer was shown to be the most effective for screening the emetic-toxin-producing B. cereus strains tested in this study. However, the ability of these PCR assays to identify emetic-toxin-producing B. cereus should also be confirmed using other methods.
KEYWORD
Bacillus cereus, PCR assay, Cereulide, Emetic toxin
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